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为明确人参内生生防细菌NJ13在宿主体内的定殖特性,该研究通过抗生素诱导获得了同时对利福平和硫酸链霉素具有稳定抗性的双抗标记菌株NJ13-R,分析了菌株在宿主体内的定殖特性,并研究了NJ13对人参黑斑病的田间防治效果。结果表明,菌株在人参根、茎、叶部均可定殖,而且定殖量与接种浓度呈正相关。菌株可在人参不同部位侵染植株并在体内进行转移,在根部和叶部定殖量表现为先升后降的变化趋势,而在茎部呈先升高后平缓趋势。研究还发现,生防细菌NJ13表现出对宿主特定部位具有一定的偏好性,随着时间推移在茎部的定殖量高于其他部位。田间试验证明,生防细菌NJ13对人参黑斑病具有较好的防治作用,其中0.76×108cfu·mL-1发酵液防效达75.62%,高于对照药剂0.67 mg·L-150%嘧菌环胺WG的防效(73.06%)。
In order to clarify the colonization characteristics of ginseng endophytic bacterium NJ13 in host, this study obtained double antibacterial marker strain NJ13-R with stable resistance to rifampicin and streptomycin sulfate through antibiotic induction. In vivo colonization characteristics, and to study the NJ13 ginseng black spot field control effect. The results showed that the strains could be colonized in the roots, stems and leaves of ginseng, and the colonization amount was positively correlated with the inoculation concentration. Strain can infect different parts of ginseng and in vivo transfer of plants, the root and leaf colonization volume showed the first increase and then decrease the trend, while the stem was first increased after the flat trend. The study also found that the biocontrol strain NJ13 showed some preference for specific sites on the host, with colonization over the stem over time. Field experiments showed that biocontrol bacteria NJ13 had a good control effect on ginseng black spot, in which the antifungal activity of 0.76 × 108 cfu · mL-1 fermentation broth reached 75.62%, which was higher than that of control agent 0.67 mg · L-150% Amine WG control (73.06%).