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采用高锰酸钾微量滴定法快速简便测定血清游离Ca2 + .最适 pH4 .2～ 7.5 ,最适温度 70～ 80° .使用该方法测定嘉陵江瓦氏黄颡鱼血清样本 ,结果表明 ,该鱼血清游离Ca2 + 在生殖间期的正常生理平均值为 8.71±2 .5 1mg/dl.在生殖周期 ,雄鱼血清游离钙水平无显著变化 (P >0 .0 5 ) ,雌鱼血清游离Ca2 + 水平随卵巢发育上升 ,产卵后逐渐下降 ,最高值 31.2 4± 15 .6 1mg/dl,最低值 6 .5 9± 1.30mg/dl.血清游离Ca2 + 在鱼类生殖期生理活动中起着重要作用
The serum free Ca2 + was determined by potassium permanganate microtitration method.The optimum pH4.2 ~ 7.5, the optimum temperature was 70 ~ 80 °.Using the method to determine the serum samples of Valinaria gladius in Jialing River, the results showed that the fish The normal physiological mean value of serum free Ca2 + in reproductive period was 8.71 ± 2.51mg / dl. There was no significant change in free calcium level in male fish during the reproductive cycle (P> 0.05), but serum free Ca2 + Level increased with ovary development and decreased gradually after oviposition, with the highest value of 31.2 4 ± 15.61 mg / dl and the lowest value of 6 .59 ± 1.30 mg / dl. Serum free Ca2 + played an important role in the physiological activities of fish in reproductive period An important role