论文部分内容阅读
作者对妊娠大鼠宫内着床部位中cAMP和cG-MP的含量进行了测定,研究采用成年雌大鼠体重200-250g,置于控制光照室内,允许随意进食及饮水,将雌鼠与雄鼠交配,以阴道中发现精子为妊娠第一天,于孕第6天以溶于0.9%NaCl的锥虫兰(1%W/V)1ml静脉注射,20分钟后用颈椎脱位处死大鼠,取出子宫,分别将兰染处(着床部位以下称IS)和未兰染处(非着床部位以下称NIS)称重,并按Gilman及Steiner方法用放射免疫测定IS和NIS中cAMP和cGMP含量。结果表明在
The content of cAMP and cG-MP in intrauterine implantation site of pregnant rats were determined. The body weight of adult female rats was 200-250g. The rats were placed in controlled light room and allowed to eat and drink at will, The mice were mated and sperms were found in the vagina. The first day of pregnancy, 1 ml of trypanosomes (1% W / V) dissolved in 0.9% NaCl was intravenously injected on the 6th day of pregnancy. After 20 minutes, the rats were killed by cervical dislocation, The uterus was removed and the blue dyeings (hereinafter referred to as “IS”) and the undamaged (hereinafter referred to as NIS) were respectively weighed and radioimmunoassayed for IS and NIS cAMP and cGMP by Gilman and Steiner content. The results show that in