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本实验包括①Ｆ３４４大鼠直接黑３８（ＤＢ３８）２００ｐｐｍ食粉饲养４ｗｋ②ＳＤ大鼠ＤＢ３８１５００ｐｐｍ食粉饲养３２ｗｋ，③同②组，但以３７５ｍｇ每ｗｋｌ次灌胃试验。各组均平行作抗菌素肠道细菌干预和单纯抗菌素而不施用ＤＢ３８的对照组，试验结束后全部内脏作组织病理学检查。４ｗｋＦ３４４大鼠ＤＢ３８饲养组显示肝内卵圆细胞和胶原纤维增生较抗菌素干扰组为严重，统计学差异极显著ｘ２＝３６．２９，Ｐ＜０．０００１），单纯抗菌素组皆正常。ＳＤ大鼠灌胃组第２４ｗｋ发生１例、第３２ｗｋ发生３例、共４例淋巴细胞样白血病。而饲养组在第２４ｗｋ、３２ｗｋ各发生１例。与单纯抗菌素对照组比较，前者有显著差异（Ｐ＜０．０５）后者差异不显著，两个相应的抗菌素干扰组皆在第３２ｗｋ各发生１例同样病变。显示抑制肠道菌可以延迟和降低ＤＢ３８导致的白血病。
This experiment included 1F344 rat direct black 38 (DB38) 200ppm feeding powder 4wk2SD rat DB381500ppm feeding powder 32wk, 3 and 2 groups, but 375mg per wkl gavage test. All groups were treated with antibacterial intestinal bacteria and antibiotics without DB38 in parallel. After the end of the experiment, all organs were examined histopathologically. 4wkF344 rat DB38 feeding group showed that the hepatic oval cells and collagen fibrosis were more severe than the antibiotic interference group, and the statistical difference was extremely significant (x2=36.29, P<0.0001). The single antibiotic group was normal. In SD rats, there was 1 case at 24wk, 3 cases at 32wk, and 4 cases of lymphoid leukemia. In the rearing group, 1 case occurred at each of 24th and 32wk. Compared with the control group with simple antibiotics, the former had significant differences (P<0.05). The latter showed no significant difference. The two corresponding antibiotic interference groups all had the same lesion at the 32nd week. It has been shown that inhibition of gut bacteria can delay and reduce DB38-induced leukemia.