目的：研究缺氧／再给氧（Ｈ／Ｒ）对中性粒细胞（Ｎｅｕ）和单核细胞（Ｍｏｎ）与诱导的培养牛脑微血管细胞粘附作用的影响．方法：牛脑微血管内皮细胞（ＣＭＥＣ）和平滑肌细胞（ＣＭＳＭＣ）经Ｈ／Ｒ处理或经ＴＮＦα和ＩＬ１α刺激，Ｍｏｎ和Ｎｅｕ与ＣＭＥＣ和ＣＭＳＭＣ粘附的细胞数目用流式细胞仪测定．结果：ＣＭＥＣ先缺氧２ｈ再经复氧与ＴＮＦα（２μｇ·Ｌ－１）作用２ｈ，Ｍｏｎ和Ｎｅｕ与ＣＭＥＣ的粘附率分别提高到３５０％±０９％和３６０％±０６％（ＴＮＦα处理组分别是２８９％±１１％和２８８％±１３％）．ＣＭＳＭＣ也按上述处理，Ｍｏｎ和Ｎｅｕ的粘附率分别显著提高为４９９％±０４％和４３９％±１４％（ＴＮＦα处理组分别为３４０％±１９％和３４０％±１３％）．ＣＭＥＣ和ＣＭＳＭＣ经ＩＬ１α刺激，可得到类似结果．结论：Ｈ／Ｒ提高Ｍｏｎ和Ｎｅｕ与ＴＮＦα和ＩＬ１α诱导的ＣＭＥＣ和ＣＭＳＭＣ的粘附作用． Objective: To investigate the effect of hypoxia / reoxygenation (H / R) on the adhesion of neutrophils (Neu) and monocytes (Mon) to cultured bovine brain microvascular cells. Methods: Bovine brain microvascular endothelial cells (CMEC) and smooth muscle cells (CMSMC) were treated with H / R or stimulated with TNFα and IL1α. The number of cells adhered by Mon and Neu to CMEC and CMSMC was determined by flow cytometry . Results: After hypoxia for 2h, the adhesion rate of Mon and Neu to CMEC increased to 35.0% ± 0.9% and 36.0% respectively after hypoxia for 2h and 2 h TNF-α (2μg · L-1) % ± 0  6% (TNF  α treatment group were 28  9% ± 1  1% and 28  8% ± 1  3%). CMSMC also according to the above treatment, Mon and Neu adhesion rates were significantly increased 499% ± 04% and 439% ± 14% (TNF  α treatment group were 34  0% ± 1  9% and 340% ± 13%). CMEC and CMSMC by IL 1α stimulation, similar results can be obtained. Conclusion: H / R can improve the adhesion of Mon and Neu with TNFα and IL1α induced CMEC and CMSMC.