论文部分内容阅读
目的探讨线粒体DNA﹙mtDNA﹚控制区T16189C变异与湖北地区妊娠糖尿病的关系。方法采用聚合酶链反应-限制性片段长度多态性,测序技术,对154例GDM患者和150例健康对照进行检测。结果 (1)GDM患者中T16189C的变异阳性者的频率显著高于变异阴性组(35.8%vs 24.3%,P=0.026);(2)GDM组中,mtDNA T16189C变异阳性者的空腹胰岛素水平和胰岛素抵抗指数(HOMA-IR)值较变异阴性者高,差异有统计学意义(P=0.042,P=0.027);多元回归分析显示该变异是参与HOMA-IR的独立变量。(3)对照组中mtDNA T16189 C变异阳性组与阴性组的年龄、性别、体重指数、血脂、空腹血糖、空腹胰岛素、HOMA胰岛素抵抗指数差异均无统计学意义(P>0.05)。结论 mtDNA T16189C变异与湖北地区GDM的胰岛素抵抗形成相关。
Objective To investigate the relationship between mitochondrial DNA (mtDNA) control region T16189C mutation and gestational diabetes mellitus in Hubei Province. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequencing were used to detect 154 GDM patients and 150 healthy controls. Results (1) The frequency of T16189C mutation in GDM patients was significantly higher than that in mutation negative patients (35.8% vs 24.3%, P = 0.026). (2) In GDM patients, the fasting insulin levels of mtDNA T16189C positive patients and insulin The resistance index (HOMA-IR) value was higher than that of the negative, the difference was statistically significant (P = 0.042, P = 0.027). Multiple regression analysis showed that the mutation was an independent variable involved in HOMA-IR. (3) There was no significant difference in age, sex, body mass index, blood lipid, fasting blood glucose, fasting insulin and HOMA insulin resistance between mtDNA T16189 C mutation positive group and negative group in the control group (P> 0.05). Conclusion The mtDNA T16189C mutation is associated with the formation of insulin resistance in GDM in Hubei Province.