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目的通过胃镜下幽门螺杆菌(Hp)检测,探讨幽门螺杆菌感染与胃十二指肠疾病的关系。方法纳入250例患者行胃镜检查,于胃窦部取活组织一块,应用荧光定量聚合酶链反应(FQ-PCR)扩增技术对幽门螺杆菌16S rRNA基因进行定量检测。结果胃、十二指肠溃疡及胃癌患者Hp阳性率高,分别为89.1%、84.2%及91.6%,50岁以上组慢性胃炎与胃、十二指肠溃疡的Hp检出率分别为65.2%和93.8%,均高于50岁以下组的50.5%和84.6%(P<0.05)。结论 Hp感染与胃炎、消化性溃疡、胃癌密切相关,FQ-PCR方法检测Hp对临床诊断和治疗意义重大。
Objective To investigate the relationship between Helicobacter pylori infection and gastroduodenal diseases by gastroscope examination of Helicobacter pylori (Hp). Methods A total of 250 patients underwent gastroscopy and a piece of living tissue was taken from the gastric antrum. The 16S rRNA gene of Helicobacter pylori was detected quantitatively by fluorescence quantitative polymerase chain reaction (FQ-PCR) amplification. Results The positive rates of Hp in gastric and duodenal ulcer patients and gastric cancer patients were 89.1%, 84.2% and 91.6%, respectively. The prevalence of Hp in patients with chronic gastritis and stomach and duodenal ulcer over the age of 50 was 65.2% And 93.8% respectively, all higher than 50.5% and 84.6% (P <0.05) of those under 50 years old. Conclusion Hp infection is closely related to gastritis, peptic ulcer and gastric cancer. The detection of Hp by FQ-PCR is of great significance in clinical diagnosis and treatment.