论文部分内容阅读
根据已发布的基因保守区域设计引物,从观赏向日葵(Helianthus annuus L.)舌状花中克隆到花青素苷生物合成途径中PAL、CHS、CHI、F3′H、DFR和ANS等6个结构基因的保守序列,序列分析表明这6个结构基因与其它植物来源的花青素苷生物合成相关基因均具有较高的同源性,分别为95%~97%、83%~99%、64%~80%、80%~82%、64%~85%和87%~89%。系统进化分析表明6个结构基因的系统进化基本上符合植物分类学分类。半定量RT-PCR分析表明,除CHS基因在管状花中未表达外,6个基因在舌状花、管状花、花蕾、叶片和茎皮中均有表达;大部分基因在花开放初期和盛期的表达较高,而花完全开放后,所有基因的表达量降低;观赏向日葵花青素苷合成相关基因的表达量在紫红色花瓣中高于红褐色花瓣,深色花瓣高于混杂色花瓣。
According to the published gene conserved regions, 6 structures of PAL, CHS, CHI, F3’H, DFR and ANS were cloned from ornamental sunflower (Helianthus annuus L.) The conserved sequence and sequence analysis showed that the six structural genes shared high homology with other plant-derived genes related to anthocyanin biosynthesis, which were 95% -97%, 83% -99%, 64 % To 80%, 80% to 82%, 64% to 85% and 87% to 89%. Phylogenetic analysis showed that the phylogenetic evolution of six structural genes was basically in accordance with the taxonomic classification of plants. Semi-quantitative RT-PCR analysis showed that 6 genes were expressed in tongue flowers, tubular flowers, flower buds, leaves and stem barks, except CHS gene was not expressed in tubular flowers; most of the genes were expressed at the beginning of flowering The expression of anthocyanin biosynthesis related genes in ornamental sunflower was higher than reddish brown petal in the purple petals and higher in the dark petals than in the hybrid petals.