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目的探讨雌激素对氧自由基(H_2O_2)所致野生型鼠成神经瘤细胞株(N2a)损伤的保护作用及机制。方法应用H_2O_2制作N2a细胞凋亡的细胞模型,采用细胞计数、酶组织化学、免疫细胞化学方法,检测在雌激素作用下,神经细胞的形态结构、细胞活性和细胞早期凋亡的相关性变化。结果细胞计数结果显示,雌激素组的细胞数量[(0.60±0.12)个/mL(×10~4)]高于其它组,雌激素加H_2O_2组的细胞数量[(0.51±0.11]×104个/mL)明显高于H2O2组[(0.30±0.09)×10~4个/mL],组间差异有统计学意义(P<0.05)。免疫细胞化学染色显示,Akt1阳性反应在雌激素组最强,在H2O2组最弱,但雌激素加H_2O_2组与正常对照组Akt1的表达没有明显差别,介于两者之间。Fasl呈相反变化。Caspase3酶活性检测结果显示,H_2O_2组Caspase3酶活性最高,雌激素加H_2O_2组Caspase3酶活性明显降低,其差别有统计学意义(P<0.01)。结论雌激素有促进N2a细胞生长的作用,并可保护H_2O_2对N2a细胞的损伤且可以对抗凋亡。
Objective To investigate the protective effect and mechanism of estrogen on the injury of wild-type neuroblastoma cell line N2a induced by oxygen free radical (H_2O_2). Methods The cell model of apoptosis of N2a cells was established by H_2O_2. The changes of the morphological structure, cell viability and early apoptosis of neurons were detected by cell counting, enzyme histochemistry and immunocytochemistry. Results The number of cells in the estrogen group was significantly higher than that in the other groups [(0.60 ± 0.12) / mL (× 10 ~ 4)] [(0.51 ± 0.11] × 104 cells / mL) was significantly higher than that in H2O2 group [(0.30 ± 0.09) × 10 ~ 4 / mL]. There was significant difference between the two groups (P <0.05). Immunocytochemical staining showed that the Akt1 positive reaction was the strongest in the estrogen group and the weakest in the H2O2 group, but there was no significant difference between the two groups in the expression of Akt1 in the estrogen plus H 2 O 2 group and the normal control group. Fasl was the opposite change. The results of Caspase3 activity showed that Caspase3 activity was the highest in H_2O_2 group and Caspase3 activity in estrogen plus H_2O_2 group was significantly lower (P <0.01). Conclusion Estrogen can promote the growth of N2a cells and protect the N2a cells against H2O2 injury.