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目的 :探讨河南林州地区贲门癌组织中 3种Ⅱ期代谢酶基因GSTM1,GSTT1及GSTP1基因多态性变化 ,进一步深入了解该地区贲门癌高易感性分子基础。方法 :利用multiplex PCR、PCR RFLP检测 19例手术切除贲门腺癌组织及 72例正常对照组织 (颊粘膜细胞和血细胞 )中代谢酶基因GSTM1、GSTT1、GSTP1多态性改变。结果 :贲门腺癌与正常对照组织中GSTM1纯合缺失基因型分别占 37%和 39% ,该基因型未明显增加对贲门癌的易感性 (OR =0 .6 9) ;GSTT1纯合缺失基因型分别占 5 3%和 39% ,该基因型可能与贲门癌易感性增高有关 (OR =2 .38) ;GSTP1的Ile/Val和Val/Val基因型合并在一起分别占 2 6 %和 4 2 % ,Val等位基因能降低个体贲门癌的易感性 (OR =0 .4 1)。结论 :GSTM1基因多态对贲门癌易感性无明显影响 ,而GSTT1和GSTP1基因多态与贲门癌易感性有关。
Objective: To investigate the changes of GSTM1, GSTT1 and GSTP1 gene polymorphisms of three kinds of stage Ⅱ metabolic enzymes in gastric cardia cancer in Linzhou, Henan Province, and to further understand the molecular basis of gastric cardia cancer susceptibility in this area. Methods: The polymorphisms of GSTM1, GSTT1 and GSTP1 were detected by multiplex PCR and PCR RFLP in 19 cases of gastric cardia adenocarcinoma and 72 cases of normal control tissues (buccal mucosa cells and blood cells). Results: The genotypes of GSTM1 homozygous deletion in gastric cardia adenocarcinoma and normal controls accounted for 37% and 39%, respectively. The genotype did not significantly increase the susceptibility to cardia cancer (OR = 0.69). The genotype of GSTT1 homozygous deletion (53%) and 39% (39%) respectively, which may be associated with the increased susceptibility of cardia cancer (OR = 2.38). The genotypes of Ile / Val and Val / Val of GSTP1 combined accounted for 26% and 4% 2%, Val allele can reduce the susceptibility of cardiac cancer (OR = 0.41). CONCLUSION: GSTM1 gene polymorphism has no obvious effect on the susceptibility to gastric cardia cancer, while the polymorphisms of GSTT1 and GSTP1 are associated with the susceptibility to gastric cardia cancer.