论文部分内容阅读
研究了以２，４－二硝基氯苯（ＤＮＣＢ）作衍生化试剂在ｐＨ９．１的硼砂缓冲液中沸水浴加热１ｈ，制得１７种ＤＮＰ－氨基酸，用于反相高效液相色谱（ＲＰ－ＨＰＬＣ）测定复方氨基酸的方法。采用Ｃ８柱，以乙腈－醋酸盐缓冲系统梯度洗脱，２２ｍｉｎ内１７种氨基酸的ＤＮＰ衍生物及衍生剂水解峰可较好分离；衍生化产物稳定，３６０ｎｍ处紫外检测，最低检出限为０．１μｇ。本法简便，重现性好，结果较为满意。
The effects of 2,4-dinitrochlorobenzene (DNCB) as derivatization reagent in boiling water bath at pH9.1 for 1 h were studied, and 17 kinds of DNP-amino acids were prepared for reversed-phase high performance liquid chromatography RP-HPLC) method for the determination of compound amino acids. Using C8 column, gradient elution with acetonitrile-acetate buffer system, the 17 amino acids of DNP derivative and derivatization peak can be separated well within 22 min. The derivatized product was stable and detected by UV at 360 nm with the detection limit of 0.1 μg. This method is simple, reproducible, the result is more satisfactory.