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目的研究过量碘性甲状腺激素代谢紊乱的机制并寻求合适的硒干预剂量。方法140只Balb/c小鼠分为7组:正常组、过量碘组(饮水含碘3000μg/L)和5个补硒组(饮水含碘3000μg/L,硒分别为0.1、0.2、0.3、0.4和0.5mg/L),共喂养16周。放射免疫法测定血清甲状腺激素水平,砷铈催化分光光度法测定尿碘和甲状腺碘水平,测定甲状腺谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和甲状腺过氧化物酶(TPO)活性以及丙二醛(MDA)水平。结果0.1～0.5mg/L补硒组甲状腺激素水平与正常组比较差异无显著性,0.2mg/L补硒组甲状腺内碘含量较过量碘组显著下降(P<0.05),0.2～0.3mg/L补硒组甲状腺GSH-Px、SOD活性和MDA水平与正常组比较差异无显著性,0.1～0.3mg/L补硒组TPO活性与正常组比较差异无显著性。结论补充硒对过量碘导致的小鼠甲状腺氧化/抗氧化水平失衡、TPO活性水平下降都有有效的干预作用。
Aim To investigate the mechanism of metabolic disorders of thyroid hormone with excess iodine and to find suitable dose of selenium intervention. Methods 140 Balb / c mice were divided into 7 groups: normal group, excess iodine group (drinking water containing 3000μg / L iodine) and 5 selenium supplementation groups (drinking water containing iodine 3000μg / L, selenium 0.1,0.2,0.3, 0.4 and 0.5 mg / L) for a total of 16 weeks. Serum thyroid hormone levels were measured by radioimmunoassay, urinary iodine and thyroid iodine levels were measured by arsenic-cerium catalytic spectrophotometry. Glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and thyroid gland Oxidase (TPO) activity and malondialdehyde (MDA) levels. Results There was no significant difference in thyroid hormone level between selenium group and selenium group (P <0.05). The level of thyroid hormone in selenium group at 0.2mg / L selenium group was significantly lower than that at iodine group (P <0.05) There was no significant difference in GSH-Px, SOD activity and MDA level between thyroid gland and selenium in selenium-enriched selenium group. There was no significant difference in TPO activity between selenium group and normal group at 0.1-0.3mg / L. Conclusion Supplemental selenium has an effective intervention on the imbalance of thyroid oxidation / anti-oxidation and the decrease of TPO activity induced by excess iodine in mice.