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作者采用葡聚糖包被活性碳饱和分析法(DCC法)分别测定了培养的人胃低分化腺癌SGC-7901细胞的胞浆及胞核KCL抽提液中的雌激素受体(ER)、孕激素受体(PR)和雄激素受体(AR)的含量。结果证明胃癌SGC-7901细胞的胞浆及胞核抽提液中ER含量为30.2和35.7fmol/mg蛋白,而PR的含量为20.3和22.7fmol/ng蛋白,但AR均为阴性(<10fmol/mg蛋白),这说明SGC-7901细胞为ER及PR阳性细胞,其ER和PR在胞浆、胞核中均有分布。提示胃癌可能为性激素依赖性肿瘤,有内分泌治疗的可能。
The authors used dextran-coated activated carbon saturation analysis (DCC) to determine the estrogen receptor (ER) in the cytoplasm and nucleus KCL extracts of human gastric poorly differentiated adenocarcinoma SGC-7901 cells. Progesterone receptor (PR) and androgen receptor (AR) levels. The results showed that the cytoplasm and nucleus extracts of gastric cancer SGC-7901 cells contained 30.2 and 35.7 fmol/mg ER, while the PR content was 20.3 and 22.7 fmol/ng protein, but the AR It is negative (<10 fmol/mg protein), indicating that SGC-7901 cells are ER and PR positive cells, and their ER and PR are distributed in cytoplasm and nucleus. It is suggested that gastric cancer may be a sex hormone-dependent tumor and there is a possibility of endocrine therapy.