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建立生物检材中度冷丁及其代谢物去甲度冷丁的GC／NPD系统分析方法，研究染毒家兔体内度冷丁的原体及代谢物分布情况，测定1例肌肉注射度冷丁过量致死者体内度冷丁和去甲度冷丁浓度。组织检材经酸水解后，在碱性条件下用乙醚提取，残余物用25μl甲醇溶解后进行气相分析。度冷丁的提取回收率高于60％，相对标准偏差小于12％；染毒家兔体内度冷丁浓度下降很快，去甲度冷丁浓度较低；度冷丁和去甲度冷丁在染毒家兔各脏器中含量分布除血液外与度冷丁过量致死者体内分布一致，尿中度冷丁和去甲度冷丁浓度最高，肝脏中浓度明显低于其它脏器。此结果为中毒检案的最佳检材选择和对毒物分析结果评价提供了科学依据。
To establish a GC / NPD system for the determination of meperidine and its metabolite norepinephrine in biopsy samples, the distribution of protamine and its metabolites in metronidazole in rabbits was studied, and one case of intramuscular injection of meperidine Body cold Ding and demetoral Ding concentration. Tissue samples were acid-hydrolyzed, extracted with ether under basic conditions, and the residue was dissolved in 25 μl methanol for gas phase analysis. The extraction and recovery rate of mechloreis was higher than 60% and the relative standard deviation (RSD) was less than 12%. The concentration of meperadd decreased rapidly in rabbits and the concentration of meperidine was lower. In addition to the content distribution of the organs in addition to blood and cold over death caused by excessive body size distribution of the body, the concentration of meperidine and demetonate in the highest concentration of cold, liver concentrations were significantly lower than other organs. This result provides the scientific basis for the best selection of the poisoning case and the evaluation of the results of poison analysis.