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目的:研究灯盏花素从细胞凋亡方面对病毒性心肌炎的治疗作用。方法:建立病毒性心肌炎的模型。40只Balb/c雄性小鼠随机分为正常对照组(n=10)、病毒感染组(n=15)和灯盏花素组(n=15),在感染后第9天处死小鼠,取其心脏进行检测。用HE染色法观察其心肌病理变化,免疫组化法观察Caspase-3情况,用TUNEL法观察心肌细胞凋亡情况。结果:①治疗组心肌病理变化改变减轻(P<0.01);②治疗组Caspase-3的表达较感染组低(P<0.05);③治疗组心肌细胞凋亡少于感染组(P<0.01)。结论:细胞凋亡在病毒性心肌炎中发挥重要作用,灯盏花素对病毒性心肌炎有治疗作用。
Objective: To study the effect of breviscapine on apoptosis in viral myocarditis. Methods: A model of viral myocarditis was established. Forty Balb/c male mice were randomly divided into normal control group (n=10), virus-infected group (n=15) and breviscapine group (n=15). The mice were sacrificed on the 9th day after infection. Its heart is tested. HE staining was used to observe the pathological changes of the myocardium. Immunohistochemistry was used to observe the status of Caspase-3. Cardiomyocyte apoptosis was observed by TUNEL method. RESULTS: 1 The pathological changes of the myocardium in the treatment group decreased (P<0.01); 2 The expression of Caspase-3 in the treatment group was lower than that in the infection group (P<0.05); 3 The apoptosis of the cardiomyocytes in the treatment group was less than that in the infection group (P<0.01). . Conclusion: Apoptosis plays an important role in viral myocarditis. Breviscapine has a therapeutic effect on viral myocarditis.