Kuhar 和Yamanura 最早使用放射自显影(Autoradiography)技术为用标记的阿片受体拮抗剂diprenorphine 及激动剂etorphine进行阿片受体定位研究。嗣后,发展了试管内标记技术,通过对阿片类、阿片肽及其它阿片样化合物的研究大大扩展了人们对阿片药物作用于神经系统的机制的了解。这种技术又使阿片受体亚型得以定位,并证实阿片受体在迷走神经纤维中的轴浆转运。阿片受体的整体标记在动物体内(整体)注入小剂量放射性标记的阿片受体拮抗剂diprenorphine 后,这种放射性结合物就可在富含阿片受体的脑区中显 Kuhar and Yamanura first used opto-autoradiography to conduct opioid receptor localization studies with the labeled opiate receptor antagonist diprenorphine and the agonist etorphine. Subsequently, in-vitro labeling techniques were developed that greatly expanded the understanding of the mechanisms by which opioids act on the nervous system through the study of opioids, opioid peptides and other opioid compounds. This technique, in turn, positions opioid receptor subtypes and confirms axonal transport of opioid receptors in vagal nerve fibers. Overall labeling of opioid receptors This radioactive conjugate can be expressed in the opioid receptor-rich brain regions by injecting a small dose of the radiolabeled opiate receptor antagonist diprenorphine (overall) into the animal body