论文部分内容阅读
本研究利用两对来源于人乳头瘤病毒Ｌ１和Ｅ１区高度保守序列引物ＧＰＬ１和ＧＰＥ１，对２０例福尔马林固定石蜡包埋组织进行ＰＣＲ分析，结果表明：以ＧＰＬ１为引物２０例口腔癌中１４例ＨＰＶ阳性；而以ＧＰＥ１为引物只有５例检测出存在ＨＰＶ感染。结果提示：ＧＰＬ１介导的ＧＰ－ＰＣＲ可有效地对福尔马林固定石蜡包埋口腔癌组织进行病毒感染流行病学回顾研究。
In this study, PCR was performed in 20 formalin-fixed paraffin-embedded tissues using two pairs of highly conserved sequence primers, GPL1 and GPE1, derived from L1 and E1 regions of human papillomavirus. The results showed that 20 cases of oral cancer with GPL1 as the primer Of the 14 cases of HPV-positive; GPE1 as a primer only 5 cases were detected HPV infection. The results suggest that GPL1-mediated GP-PCR can effectively retrospectively study the viral infection in formalin-fixed paraffin-embedded oral cancer tissues.