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目的证明红细胞免疫(C3b)检测技术应用于体检中,对判断人类是否处于亚健康状态具有重要的意义.方法取体检者中319例健康和亚健康的标本用卡式微柱凝胶法检测.结果亚健康研究组比健康正常对照组红细胞表面补体C3b水平出现明显降低,高血脂、长期吸烟、酗酒、自诉易感冒组与正常对照组比较,差异有显著性(P<0.05);乙肝病毒携带组、老年组与正常对照组比较,差异有非常显著性(P<0.01).结论红细胞免疫在机体抵抗疾病过程及许多疾病免疫发病机理中占有重要的地位,当机体出现亚健康状态时,由于各种原因影响了红细胞识别、粘附、储存、杀伤抗原、清除循环免疫复合物等功能时,红细胞表面的C3b受体的活性就会出现降低,此时应引起重视,及时纠正红细胞免疫功能降低,对抗病抗衰老起着重要作用.“,”objective: to apply erythrocyte immune detection technique in physical examination is of vital significance in judging whether humans are in the state of sub-health. Method: by using micro-column gel technique (MGT) to detect the 319 samples from healthy and sub-healthy physical examinees. Result: compared with the contrast group, the level of C3b of erythrocyte complement becomes obviously lower, and compared with the contrast group, there was significant difference in the groups of hyperlipemia, chronic smokers and alcoholics and self-diagnosed easy cold-catchers(P<0.05); compared with the contrast group, there was significant difference in the asymptomatic carriers of HBV group and the aged group(P<0.01). Conclusion: erythrocyte immunity plays a significant role in the process of organism resisting against ilnesses and in the immune mediated mechanism of many ilnesses. When organism is in the state of sub-health, due to various reasons, the blood’s functions of recognition, adherence, storage, the kiling of antigen and the clearance of circulating immunocomplex are affected, which wil lead to the lowering of activity of the C3b receptor on the surface of erythrocyte. When this happens, to remedy the decrease of the erythrocyte immune function wil be of vital importance in resisting ilnesses and aging.