论文部分内容阅读
从自然发病的病蚌中提取出病毒,经人工感染健康三角帆蚌后呈现出典型的病理变化。组织学观察显示消化腺、胃、肠、外套膜、斧足和鳃等均出现大量细胞空泡化、部分细胞肿大、上皮细胞排列不紧密,结缔组织不完整,部分细胞溶解甚至脱落等,表明它们均为病毒损害的主要靶器官。透射电镜观察显示大量砂样病毒存在于患病三角帆蚌不同器官中。病毒粒子为直径约120 nm的圆球形,表面具有明显的纤突,无囊膜结构。被感染细胞呈现明显的病理变化,包括细胞质大片空泡化,细胞器相对减少或缺失,细胞核电子密度加深,肠黏膜上皮细胞不同程度的变性和坏死,消化腺细胞核变形肿大,鳃小片上皮细胞坏死脱落,颗粒细胞的核膜溶解等。
The virus was extracted from the naturally occurring disease clam and showed typical pathological changes after the artificial infection of the healthy clam. Histological observations showed that a large number of cells in the digestive gland, stomach, intestine, mantle, axillary and gills were vacuolized, part of the cells were enlarged, the epithelial cells were not arranged closely, the connective tissue was incomplete, some of the cells were dissolved or even shed, All of them are the main target organs for virus damage. Transmission electron microscopy showed that a large amount of sand-like virus existed in different organs of diseased Hyriopsis cumingii. The virus particle is a round sphere with a diameter of about 120 nm. The surface of the virus particle has obvious fiber processes and no capsule structure. Infected cells showed obvious pathological changes, including large cytoplasm vacuolization, relative decrease or deletion of organelles, deepening electron density of the nucleus, varying degrees of degeneration and necrosis of intestinal mucosal epithelial cells, enlargement of digestive glandular nucleus, gill epidermal cell necrosis Shedding, granulosa cell nuclear membrane lysis and so on.