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[Objective] To research the antibacterial activity of water extracts from RADIX FICI HIRTAE on the food-induced pathogenic bacteria, and to compared with the traditional antibacterial methods. [Method] With RADIX FICI HIRTAE as the materials, antibacterial activity of water extracts from RADIX FICI HIRTAE was researched by chromotest microassay, filter paper dispersion method and doubling dilution method. [Result] The optimal condition of methylthiazol tetrazoliam (MTT) method was 1×105-1×106 CFU/ml initial concentration of bacterial solution, and 0.5 mg/ml final concentration of MTT. Under this condition, water extracts of RADIX FICI HIRTAE showed antibacterial activity against Escherichia coli. and Staphylococcus aureus; and the minimal inhibitory concentrations were 11.21 and 14.07 mg/ml, respectively. Results of filter paper dispersion method and doubling dilution method showed that water extracts of RADIX FICI HIRTAE had antibacterial activity. [Conclusion] Compared with the traditional antibacterial methods, MTT method had the advantages of high sensitivity, which was more suitable for the antibacterial activity detection of plant extracts with deep color.
[Objective] To research the antibacterial activity of water extracts from RADIX FICI HIRTAE on the food-induced pathogenic bacteria, and to compared with the traditional antibacterial methods. [Method] With RADIX FICI HIRTAE as the materials, antibacterial activity of water extracts from RADIX FICI HIRTAE was researched by chromotest microassay, filter paper dispersion method and doubling dilution method. [Result] The optimal condition of methylthiazol tetrazolium (MTT) method was 1x105-1x106 CFU / ml initial concentration of bacterial solution, and 0.5 mg / ml final concentration of MTT. Under this condition, water extracts of RADIX FICI HIRTAE showed antibacterial activity against Escherichia coli. and Staphylococcus aureus; and the minimal inhibitory concentrations were 11.21 and 14.07 mg / ml, respectively. Results of filter paper dispersion method and doubling dilution method showed that water extracts of RADIX FICI HIRTAE had antibacterial activity. [Conclusion] Compared with the tra ditional antibacterial methods, MTT method had the advantages of high sensitivity, which was more suitable for the antibacterial activity detection of plant extracts with deep color.