论文部分内容阅读
目的利用pEGFP-N1质粒转染鼻咽癌细胞株,建立稳定表达绿色荧光蛋白的鼻咽癌细胞株CNE2-EGFP,为整体鼻咽癌可视化研究提供良好的试验材料。方法利用脂质体转染法,将pEGFP-N1质粒转染鼻咽癌细胞株,通过G418抗性筛选、亚克隆扩增获得GFP稳定表达的人鼻咽癌细胞株CNE2-EGFP。结果成功获得多个稳定表达GFP的人鼻咽癌细胞CNE2-EGFP细胞株,比较CNE2和CNE2-EGFP发现,两者生长曲线、细胞周期分布、裸鼠成瘤能力等均无显著性差异。结论建立了非G418依赖、稳定表达GFP且保持母株细胞特性的人鼻咽癌细胞株CNE2-EGFP,为鼻咽癌整体可视化研究打下了坚实的基础。
Objective To construct NPC cell line CNE2-EGFP stably expressing green fluorescent protein (EGFP) by transfecting NPC cell line with pEGFP-N1 plasmid, and to provide a good experimental material for the overall visualization of nasopharyngeal carcinoma. Methods The plasmid pEGFP-N1 was transfected into nasopharyngeal carcinoma cells by lipofection method. The GFP-resistant human nasopharyngeal carcinoma cell line CNE2-EGFP was obtained by G418 screening and subcloning. Results A number of CNE2-EGFP cell lines were successfully obtained from human nasopharyngeal carcinoma cells stably expressing GFP. Compared with CNE2 and CNE2-EGFP, there was no significant difference in the growth curve, cell cycle distribution and tumorigenicity of nude mice. Conclusions The human nasopharyngeal carcinoma cell line CNE2-EGFP that is non-G418-dependent, stable expression of GFP and maintains the characteristics of the mother plant cells has been established, laying a solid foundation for the overall visualization of nasopharyngeal carcinoma.