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目的探讨肺癌动物模型的建立及其对药物治疗的反应。方法 160只C57BL/6小鼠随机分为胸腔移植组和皮下移植组,每组80只。在小鼠腋后线第6肋间胸腔或小鼠腋部皮下,分别移植鼠源性Lewis肺癌细胞0.2ml(5×106/ml)。采用不同化疗药物治疗后,观察小鼠生存状态,用免疫组化方法检测肿瘤组织内增殖细胞核抗原(PCNA)和血管内皮生长因子(VEGF)蛋白表达。结果与皮下移植组比较,胸腔移植组小鼠治疗后生活状态好转,VEGF表达和PCNA阳性细胞数明显下降。结论与小鼠皮下移植肺癌模型比较,胸腔移植模型能更好的反映药物的作用。
Objective To investigate the establishment of an animal model of lung cancer and its response to drug therapy. Methods 160 C57BL / 6 mice were randomly divided into thoracic cavity transplantation group and subcutaneous transplantation group, 80 rats in each group. Murine Lewis lung cancer cells (0.2 ml, 5 × 10 6 / ml) were transplanted subcutaneously in the 6th intercostal space of the axillary line of mice or subcutaneously in mouse axilla. After treatment with different chemotherapeutic drugs, the survival status of the mice was observed, and the expression of PCNA and VEGF protein in tumor tissues was detected by immunohistochemistry. Results Compared with the subcutaneous transplantation group, the life condition of the mice in the thoracic transplantation group improved, and the expression of VEGF and the number of PCNA positive cells decreased obviously. Conclusion Compared with the subcutaneous lung cancer model in mice, the model of thoracic cavity transplantation can better reflect the effect of drugs.