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目的 探讨细胞粘附分子和皮肤移植后排异反应之间的关系。方法 用ＨＥ及免疫组化方法观察２０例大鼠Ⅲ度烫伤异体－自体皮肤混合移植和１５例同种大鼠大张异体皮肤移植组织在移植后４～５，７，１４，２１和２８ｄ时细胞粘附分子ＩＣＡＭ－１和ＬＦＡ－１的表达情况。结果 大鼠Ⅲ度烫伤后异体－自体皮肤混合移植组织中ＬＦＡ－１＋淋巴细胞和ＩＣＡＭ－１＋细胞的表达均低于同种大张异体皮肤移植组织，与排异程度相一致，分别在移植后７和１４ｄ时差异显著（Ｐ＜０．０５）。结论 ＩＣＡＭ－１／ＬＦＡ－１不是主要促使皮肤混合移植排异反应中Ｔ细胞活化的共刺激信号，但ＩＣＡＭ－１＋细胞和ＬＦＡ－１＋淋巴细胞在介导同种大张异体皮移植排异反应中起主要作用。
Objective To investigate the relationship between cell adhesion molecules and rejection after skin transplantation. Methods Hematoxylin and eosin staining (HE) and immunohistochemical methods were used to observe the effects of allograft-autologous skin grafting combined with third degree scald and skin allograft in 15 rats on 4, 5, 7, 14, 21 and 28 days after transplantation Cell adhesion molecules ICAM-1 and LFA-1 expression. Results The expression of LFA-1 + lymphocytes and ICAM-1 + cells in allogeneic-autologous skin mixed transplantation group was lower than that in the same large allotransplanted skin tissue after Ⅲ degree scald in rats, which was consistent with the degree of rejection after transplantation There was a significant difference between 7 and 14 days (P <0.05). Conclusion ICAM-1 / LFA-1 is not the costimulatory signal that mainly promotes the T cell activation in skin allograft rejection. However, ICAM-1 + cells and LFA-1 + lymphocytes play an important role in mediating allograft rejection Play a major role.