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2012年采用PCR-DGGE法对云南省典型高原湿地——剑湖地区的典型水生植物茭草的根际土壤固氮菌多样性进行分析。结果显示,入水口永丰河的茭草根际土壤固氮菌Shannon-Wiener指数和Simpson丰富度最高,出水口黑惠河的茭草根际土壤固氮菌Shannon-Wiener指数和Simpson丰富度最低。典型对应分析表明,NH4+和NO3-显著影响根际土壤固氮菌群落结构。同源比对分析显示,所有序列共分为2个类群,其中有11条带属于Betaproteobacteria,占84.6%;2条带属于Gammaproteobacteria,占15.3%。聚类分析结果表明,大部分固氮菌(84.6%)属于红环菌目,少部分(15.3%)属于着色菌目。
In 2012, the diversity of rhizosphere soil nitrogen-fixing bacteria in the typical aquatic plant Hsipalpis gracilis, a typical wetland in Jian-Hu area of Yunnan Province, was analyzed by PCR-DGGE. The results showed that the Shannon-Wiener index and Simpson abundance of Azotobacter in the Rhizosphere of Penaeidula australis at the water inlet were the highest, and the Shannon-Wiener index and Simpson abundance of Azotobacter in the Rhizosphere of Penaera chinensis at the outlet were the lowest. The typical corresponding analysis showed that NH4 + and NO3- significantly affected the structure of Azotobacter in the rhizosphere soil. Homologous alignment analysis showed that all the sequences were divided into two groups, of which 11 belonged to Betaproteobacteria, accounting for 84.6%; two belonged to Gammaproteobacteria, accounting for 15.3%. Cluster analysis showed that most Azotobacter species (84.6%) belong to the order of Rhodocytheria, and a small proportion (15.3%) belong to the order of the colored bacteria.