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植物GH3基因家族具有生长素(IAA)等植物激素的氨基酸化合成酶活性,参与调节IAA等的动态平衡,在植物生长发育和抗逆性方面具有重要作用。为了明确大豆GH3基因家族成员对主要农药的响应特点,用qRT-PCR分析了20种不同作用机制的农药对该家族XM003518758、XM003521754和XM003543231在60 min内的诱导特点。结果表明,XM003518758基因的表达受草甘膦、氰戊菊酯轻微抑制,受2甲4氯等其余18种农药诱导上调但不超过2.5倍。所有测试的20种农药均不抑制XM003521754基因的表达,而最大诱导表达量达10倍以上。2甲4氯、乙草胺、百草枯、氰戊菊酯和戊唑醇对XM003543231诱导表达超过50倍,而其余15种测试农药对XM003543231的表达无抑制效应。而大豆XM003521754基因只受2甲4氯、二氯喹啉酸和多菌灵的显著诱导上调。
The plant GH3 gene family has the amino acid synthase activity of plant hormones such as IAA and plays an important role in the dynamic balance of IAA and other plant growth and stress resistance. In order to clarify the response of soybean GH3 gene family members to major pesticides, the induction characteristics of the families XM003518758, XM003521754 and XM003543231 within 60 min were analyzed by qRT-PCR using 20 pesticides with different mechanism of action. The results showed that the expression of XM003518758 gene was slightly inhibited by glyphosate and fenvalerate, and was up-regulated but not more than 2.5-fold by the remaining 18 pesticides, such as 2-methyl-4-chloro. All 20 pesticides tested did not inhibit the expression of XM003521754 gene, and the maximum induced expression of more than 10 times. Methyl chloride, acetochlor, paraquat, fenvalerate and tebuconazole induced more than 50-fold expression of XM003543231, while the remaining 15 tested pesticides had no inhibitory effect on the expression of XM003543231. However, the soybean XM003521754 gene was only significantly upregulated by 2-MCPD, quinclorac and carbendazim.