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根际细菌丰富多样,对植物的生长发育有重要影响。为更好的了解野生蒙桑和移植栽培蒙桑根际细菌的多样性组成和差异,本研究提取了两样本的宏基因组DNA,利用Roche 454 GS FLX测序技术对样本菌群的16S r RNA基因的V1~V3区域进行测序。测序结果表明:野生蒙桑根际细菌的主导类群为变形菌门(31.62%)和酸酐菌门(19.8%);栽培蒙桑的主导类群为厚壁菌门(89.07%),两样本的沙浓指数分别为5.8和1.33。栽培蒙桑样本OTU498的基因序列数占总样本的78.9%,其最相近菌属为苏云金芽孢杆菌;野生蒙桑OTU656、OTU556、OTU568和OTU665占总样本的8.17%,最相近菌属是丝状共生菌。进化分析发现两样本菌群具有各自的特异性,大都来源于同一菌门的不同菌属,分别聚类。本研究表明移栽后蒙桑根际细菌的多样性降低,主导类群也发生了变化,基于16S r RNA测序可以揭示根际细菌的组成结构。
Rhizosphere bacteria rich and diverse, have a significant impact on plant growth and development. In order to better understand the diversity and composition of the bacteria in the rhizosphere of Mongolian mulberry and transplanting cultivated M. mongolica, two genomic DNAs were extracted. The Roche 454 GS FLX sequencing technique was used to analyze the 16S rRNA gene V1 ~ V3 region for sequencing. The results showed that the dominant bacteria in the rhizosphere of Morus alba were 31.62% and 19.8%, respectively. The dominant species in the cultivated Mengsan were 89.07% The concentration index is 5.8 and 1.33 respectively. The gene sequence number of mulberry cultivars OTU498 accounted for 78.9% of the total samples, and the closest strain was Bacillus thuringiensis. The wild mulberry OTU656, OTU556, OTU568 and OTU665 accounted for 8.17% of the total samples. Symbiotic bacteria. Evolutionary analysis found that the two samples have their own specificities of bacteria, mostly from the same fungi different genus, respectively, clustering. This study showed that the diversity of bacteria in the rhizosphere of M. mongolica decreased after transplanting, and the dominant groups also changed. Based on the 16S rRNA sequencing, the composition of rhizobacteria could be revealed.