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利用Stock6与甜玉米自交系265OR1的29个F2诱导株对2个美国超甜玉米单交种和2个人工合成超甜玉米群体进行杂交诱导,通过子粒颜色和形态标记识别,结合田间鉴定筛选单倍体,利用0.05%秋水仙素+2%二甲基亚砜进行染色体加倍。结果表明,12个诱导株诱导出单倍体,共获得19株单倍体植株,平均单倍体诱导率为0.016%～0.237%,其中诱导株3-2、3-6和2-7诱导率相对较高。不同类型材料间被诱导频率差异明显,遗传基础复杂、有利基因频率相对较高的群体被诱导率显著高于单交种,相同类型材料间被诱导率差异不显著。经染色体加倍,成功获得4株双单倍体,加倍率21.05%。
29 F2-inducing plants from Stock6 and sweet maize inbred lines 265OR1 were used to induce hybridization of two super-sweet maize hybrids and two super-sweet synthetic maize hybrids. Through the identification of seed color and morphological markers, Chromosome doubling with 0.05% colchicine + 2% dimethyl sulfoxide. The results showed that 12 haploid plants were induced from 12 induced plants and 19 haploid plants were obtained. The average haploid induction rate was 0.016% -0.237%. Among them, induced plants 3-2, 3-6 and 2-7 were induced The rate is relatively high. Different types of materials were significantly different frequency of induction, the genetic basis of complex, favorable gene frequency of the population was significantly higher than the single cross-species, the same type of material induction rate was not significantly different. After chromosome doubling, 4 double haploid plants were successfully obtained, with a doubling rate of 21.05%.