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参考汉坦病毒（ＨＶ）的基因文库软件设计Ｍ片段Ｇ－１区型特异性引物，以ＨＶ ７６／１１８、Ｈ－１１４、Ａ－９及Ｒ－｛２２｝株ＲＮＡ为阳性模板，建立ＨＶ的分型方法──逆转录一半巢式聚合酶链反应（ＲＴ－ｈｅｍｉｎｅｓｔｅｄ ＰＣＲ），对湖北省不同地区分离的３０株ＨＶ进行分 型。结果显示：（１）建立的ＲＴ－ｈｅｍｉｎｅｓｔｅｄ ＰＣＲ分型方法对ＨＶ两型的代表株７６／１１８（ＨＴＮ）、Ａ－９（ＨＴＮ）、Ｈ－１１４（ＨＴＮ）及Ｒ－｛２２｝（ＳＥＯ）ＲＮＡ进行了特异性扩增，其大小与理论值一致；此 法只能检测ＨＶ ＲＮＡ，不能检测其它病毒ＲＮＡ。（２）分离于湖北省不同地区的３０株ＨＶ的分型结果 为汉滩型２１株、汉城型９株，其中长江以南汉滩型１２株、汉城型２株；长江以北汉滩型９株、 汉城型７株。这表明建立的ＲＴ－ｈｅｍｉｎｅｓｔｅｄ ＰＣＲ用于ＨＶ的检测特异性好；分析湖北省不同 地区分离的３０株ＨＶ，显示湖北省ＨＦＲＳ流行为混合疫区；且在湖北地区具有一定的地理聚集性。
The M segment G-1 region-specific primers were designed with reference to the gene library software of Hantavirus (HV), and the HV HVs were identified as HV 76/118, H-114, A-9 and R- {22} (RT-heminested PCR), 30 HV isolates from different regions of Hubei Province were classified. The results showed that: (1) The RT-heminested PCR typing method was successfully used to detect HVNH, HNN, HNR, HNR, ) RNA specific amplification, its size and theoretical value; this method can only detect HV RNA, can not detect other viral RNA. (2) The genotyping results of 30 HV isolates from different regions in Hubei Province were 21 for Hantaan type and 9 for Seoul type, of which 12 were Hantan type in the south of Yangtze River and 2 in Seoul type. 9 strains, Seoul type 7 strains. This indicates that the established RT-heminested PCR has good specificity for the detection of HV. Analysis of 30 HVs isolated from different areas in Hubei Province shows that the epidemic of HFRS in Hubei is mixed epidemic-prone areas and has some geographical aggregation in Hubei Province.