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目的 :观察血小板源性生长因子 ( PDGF)对于培养脐静脉内皮细胞碱性成纤维细胞生长因子 ( b FGF)水平的影响 ,揭示 PDGF促进新生血管形成的机制。方法 :免疫组织化学检测培养脐静脉内皮细胞 b FGF水平。结果 :缺氧培养与常规培养相比内皮细胞胞浆b F GF水平无显著变化 ;不同剂量的 PDGF在常规或缺氧培养条件下均能增加内皮细胞胞浆 b FGF水平 ,且呈剂量依赖性。结论 :说明 PDGF可能通过直接促血管形成生长因子的介导作用间接促进新生血管的形成。
Objective: To observe the effect of platelet-derived growth factor (PDGF) on the level of basic fibroblast growth factor (bFGF) in cultured human umbilical vein endothelial cells and to reveal the mechanism of PDGF promoting neovascularization. Methods: Immunohistochemistry was used to detect the bFGF level in cultured human umbilical vein endothelial cells. Results: There was no significant difference in the level of bFGF in endothelial cells between hypoxia and normal culture. Different doses of PDGF could increase the level of bFGF in endothelial cells in a dose-dependent manner under both routine and hypoxic conditions . Conclusion: PDGF may indirectly promote neovascularization through the direct mediation of angiogenic growth factors.