次代细胞培养（ｓｅｃｏｎｄａｒｙｃｅｌｌｃｕｌｔｕｒｅｓ）：将原代细胞培养物用胰蛋白酶或螯合剂ＥＤＴＡ处理，使原代细胞从容器壁上脱落并分散成单个细胞。做成细胞悬液，分瓶培养后，再次长成单层细胞者，称为次代细胞培养，其生物学特性及对病毒的易感性大致与原... Secondary cell cultures: primary cell cultures are treated with trypsin or a chelator EDTA to detach primary cells from the walls of the container and disperse into individual cells. Made into cell suspension, sub-bottle culture, once again grown into single-layer cells, known as the second-generation cell culture, its biological characteristics and susceptibility to the virus roughly with the original ...