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目的探讨临床生物化学实验室用水按微生物监测分级使用的重要性。方法水质微生物培养在一级标准和二级标准两种条件下,以二氧化碳浓缩试剂为测定试剂比较不同微生物培养结果时对校准工作曲线的影响;比较使用浓缩试剂和非浓缩试剂对测定二氧化碳的影响。用注射用水和实验室水机所制纯水分别复溶甘油三酯干粉试剂后,观察试剂颜色变化和试剂空白吸光度,比较不同实验室试剂用水等级对干粉试剂的影响。结果微生物培养结果为一级标准的纯水,可以用于试剂溶剂和仪器清洗,微生物培养结果为二级标准的纯水,不能作为试剂溶剂,只能用于设备清洗。注射用水可用于复溶甘油三酯干粉试剂,本实验纯水机制备的纯水不适用于复溶甘油三酯干粉试剂。结论建议医学实验室每天监控纯水机电阻率,每月对纯水机水箱进行微生物培养,不同等级纯水用于不同实验室操作。医学实验室用水定期检测(微生物含量、pH值范围等)对于实验室风险评估、保证实验室质量非常必要。
Objective To investigate the importance of clinical biochemical laboratory water monitoring and grading by microorganisms. Methods Microbial culture of water quality Under the first and second standard conditions, the effect of different concentration of carbon dioxide enrichment reagent on the calibration curve was compared with that of carbon dioxide concentration reagent. The influence of using concentrated reagent and non-concentrated reagent on the determination of carbon dioxide . The injection of water and laboratory water were made of pure water were reconstituted triglycerides dry powder reagent, the color change and the reagent blank absorbance observed, compare the different laboratory reagent water grade on dry powder reagents. Results The result of the culture was Grade I standard pure water, which could be used for reagent solvent and instrument cleaning. The result of microbial culture was Grade II standard pure water. It could not be used as reagent solvent and only used for equipment cleaning. Water for injection can be used to reconstitute triglyceride dry powder reagent, pure water prepared in this experiment pure water is not suitable for reconstitution triglyceride powder reagent. Conclusion It is suggested that the medical laboratory should monitor the resistivity of the pure water machine every day and conduct microbiological culture on the pure water tank every month. Different grades of pure water are used in different laboratory operations. Medical laboratory water testing (microbial content, pH range, etc.) Laboratory risk assessment to ensure the quality of the laboratory is necessary.