二十年生的柠檬桉顶芽,培养在附加泛酸钙、生物素 BAP 和 KT 的 Ms 培养基上,形成了大量的苗。这些苗用 NAA,经过三次继代培养后能诱导出根。对于顶芽最初诱导出的苗,在15℃培养室,采用连续光照,应用液体转动培养,对其生长发育是必需的。但是,这些条件对以后的继代培养或以实生苗为材料是不需要的。至今已进行了15代继代培养,而在每次继代培养中,都得到了健康而又具生活力的植株。估计使用这种方法从柠檬桉成年树一个芽,一年内可以得到100,000株苗木。 Twenty-year-old lemon eucalyptus buds were cultured on Ms medium supplemented with calcium pantothenate, biotin BAP and KT to form a large number of shoots. These seedling NAA, after three subcultures can induce root. For the seedlings initially induced by the terminal bud, it is necessary for them to grow and develop in a 15 ° C culture room using continuous light and rotating with liquid. However, these conditions are not required for subsequent subcultures or seedlings. To date, 15 generations of subculture have been carried out, and in each subculture, healthy and viable plants have been obtained. It is estimated that by using this method, a bud can be obtained from adult tree of lemon eucalyptus and 100,000 seedlings can be obtained within a year.