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为研究妊娠期暴露于900 MHz电磁辐射对子代大鼠学习记忆能力损伤的影响及机制,将32只清洁级Wistar孕鼠随机分为低(10 min)、中(60 min)、高(120 min)剂量辐射组和对照(假暴露)组,每组8只,孕鼠自妊娠第1天暴露于900MHz电磁辐射,每日3次,直至仔鼠出生。于仔鼠出生后28 d进行Morris水迷宫试验,观察仔鼠学习记忆能力。采用Western blot法检测仔鼠海马神经磷脂酶Cγ(PLCγ)和细胞外调节蛋白激酶(ERK1/2)的蛋白表达水平。结果显示,与对照组比较,仅高剂量辐射组仔鼠逃避潜伏期延长,穿越平台次数减少;中、高剂量辐射组仔鼠海马神经元PLCγ磷酸化、ERK1/2磷酸化降低,差异均有统计学意义(P<0.05)。妊娠期暴露于900 MHz电磁辐射对子代大鼠学习记忆能力具有损伤作用,这可能与其抑制PLCγ和ERK1/2活化有关。
In order to study the effect and mechanism of exposure to 900 MHz electromagnetic radiation on learning and memory abilities in offsprings during pregnancy, 32 pregnant Wistar pregnant rats were randomly divided into low (10 min), medium (60 min), high (120 min) and control (sham) groups, 8 rats in each group. The pregnant rats were exposed to 900MHz electromagnetic radiation on the first day of pregnancy three times a day until the offspring were born. Morris water maze test was carried out on the pups 28 days after birth to observe the learning and memory abilities of the offspring. Western blot was used to detect the protein expression levels of neuronal phospholipase Cγ (PLCγ) and extracellular regulated protein kinase (ERK1 / 2) in the hippocampus. Compared with the control group, only high-dose radiation group prolonged the escape latency and decreased the number of crossing the platform; PLCγ phosphorylation and phosphorylation of ERK1 / 2 in the hippocampal neurons of the medium and high dose radiation groups decreased, with statistical differences Significance (P <0.05). Gestational exposure to 900 MHz electromagnetic radiation has impaired learning and memory abilities in offspring rats, which may be related to its inhibition of PLCγ and ERK1 / 2 activation.