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目的检测郑氏植物蛋白片段(Zheng’s plant protein segment,ZPPS)在乳腺癌中的表达,探讨其临床病理联系及其与Her-2基因表达的相关性。方法应用免疫组化SP法检测51例乳腺癌与23例正常乳腺组织中ZPPS的表达;应用FISH技术检测Her-2基因在乳腺癌中的表达。结果正常乳腺组织中,8例ZPPS呈阳性表达,阳性率为34.78%;乳腺癌组织中,38例ZPPS呈阳性表达,阳性率为74.51%,二者表达差异有统计学意义(P<0.05)。51例乳腺癌中Her-2基因扩增率为39.22%,ZPPS阳性表达与FISH检测Her-2基因扩增呈负相关(P<0.05)。ZPPS的表达与淋巴结转移呈负相关(P<0.05)。结论标记ZPPS对乳腺癌的诊断及判定淋巴结转移具有实用价值。因在乳腺癌中ZPPS的表达与Her-2基因扩增呈负相关,所以临床病理工作中可选择ZPPS阴性病例进行FISH检测,从而减少盲目进行FISH基因扩增和Herceptin分子靶向治疗的病例。
Objective To detect the expression of Zheng’s plant protein segment (ZPPS) in breast cancer, and to explore its clinicopathological relationship and its relationship with Her-2 gene expression. Methods Immunohistochemical SP method was used to detect the expression of ZPPS in 51 cases of breast cancer and 23 cases of normal breast tissues. The expression of Her-2 gene in breast cancer was detected by FISH. Results The positive rate of ZPPS in normal breast tissue was 8 cases (34.78%). In the breast cancer tissues, 38 cases of ZPPS were positive, the positive rate was 74.51%, the difference was statistically significant (P <0.05) . The amplification of Her-2 gene in 51 cases of breast cancer was 39.22%. The positive expression of ZPPS was negatively correlated with the amplification of Her-2 gene by FISH (P <0.05). The expression of ZPPS was negatively correlated with lymph node metastasis (P <0.05). Conclusion The marker ZPPS has practical value for the diagnosis of breast cancer and the identification of lymph node metastasis. Because of the negative correlation between the expression of ZPPS and the amplification of Her-2 gene in breast cancer, we selected FISH detection of ZPPS-negative cases in clinicopathology to reduce the blind cases of FISH gene amplification and Herceptin molecular targeted therapy.