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目的研究不同力值作用下牵张成骨区内实验牙龈沟液(gingival crevicular fluid,GCF)中天冬氨酸转氨酶(aspartate aminotransferase,AST)动态变化。方法将8只Beagle犬依据牵张成骨后移动实验牙所用力值分为50 g组和100 g组,每组4只。各组右侧以相应力值将实验牙向远中位置移进骨再生区域,将各组左侧设置为研究对照,不予加力。对比分析加力各时点(加力前、后、1d、2d、3d、7d、14d、28d)实验牙GCF中AST动态变化。结果各组不同时点实验牙GCF中AST动态变化明显(P<0.01),各组近远中侧的GCF中AST动态变化明显(P<0.01);受力后50 g组与100g组表现为下降趋势,在21 d回归为基线水平;受力后24 h,100 g组的AST表现为下降趋势,在7 d后表现为低水平维持;予以定时分析发现,100 g组AST在基线水平趋势以下维持当21 d时可呈恢复表现,但随后又呈上升趋势。结论在实施牵张成骨治疗患者的牙周组织以及细胞中,AST的活性改变及分布,同正畸力的施加时间、大小、作用部位高度相关。AST在施加100 g力时,AST波动变化呈稳定状态,能为临床判定正畸加力预期效果提供重要参考。
Objective To study the dynamic changes of aspartate aminotransferase (AST) in experimental gingival crevicular fluid (GCF) under distraction osteogenesis. Methods Eight Beagle dogs were divided into 50 g group and 100 g group according to the force value of distraction osteogenesis after moving the experimental teeth, 4 rats in each group. On the right side of each group, the experimental tooth was moved to the bone regeneration area with the corresponding force values, and the left side of each group was set as the research control without applying force. The dynamic changes of AST in experimental teeth at different time points (before, after, 1d, 2d, 3d, 7d, 14d, 28d) were compared and analyzed. Results The dynamic changes of AST in GCF at different time points in each group were significant (P <0.01), and the dynamic changes of AST in GCF in each group were significant (P <0.01). After 50 g and 100 g And decreased to the baseline level at 21 d. After 24 h of stress, AST in 100 g group showed a downward trend and maintained at a low level after 7 days. Timing analysis showed that AST in 100 g group showed a trend toward baseline The following maintained when the 21d when the recovery performance, but then on the rise. Conclusion The changes and distribution of AST activity in periodontal tissues and cells in patients undergoing distraction osteogenesis are highly correlated with the time, size and site of action of orthodontic force. AST exert 100 g force, AST fluctuations showed a steady state, which can provide an important reference for the clinical judgment of orthodontic force expected results.