目的　观察西立伐他汀对内皮细胞一氧化氮合酶 (NOsythase ,eNOS)和细胞间黏附分子 1 (intercellularadhesionmolecule 1 ,ICAM 1 )基因的表达 ,NOS活力和黏附的THP 1细胞量的影响。方法　以氧化LDL抑制培养的ECV 3 0 4细胞表达eNOS ,加入不同浓度西立伐他汀后 ,用RT PCR法检测eNOSmRNA ,硝酸还原酶法测定培养基中NO量。以脂多糖 (LPS)及西立伐他汀加入ECV 3 0 4细胞后 ,用RT PCR法检测ICAM 1mRNA ,并测定黏附的THP 1细胞量。结果　随着西立伐他汀浓度增加 ,内皮细胞eNOSmRNA水平增加 ,0 0 1 ,0 1 ,1 0 μmol/L时分别增加 5 0 %,1 5 0 %,3 0 0 %,P均 <0 0 5。培养液中NO亦相应增加 ,0 0 1 ,0 1 ,1 0 μmol/L时分别增加 2 6 7%,92 3 %,2 3 0 %,P <0 0 5。西立伐他汀浓度为 0 1 ,1 0 μmol/L时 ,ICAM 1mRNA分别从 70 7± 1 0 4降至 5 6 2± 6 5 ,3 5 8± 4 2 ,P <0 0 5。黏附于ECV 3 0 4细胞的THP 1细胞在 1 0 μmol/L时被抑制 2 6 %,P <0 0 5。结论　西立伐他汀能诱导内皮细胞eNOS基因的表达及增加NOS活力 ;抑制内皮细胞表达ICAM 1mRNA及THP 1细胞黏附于内皮细胞 Objective To observe the effect of cerivastatin on the expression of NO synthase (eNOS) and intercellular adhesion molecule 1 (ICAM 1), the activity of NOS and the amount of adherent THP 1 in endothelial cells. Methods The expression of eNOS in cultured ECV-304 cells was induced by oxidized LDL. After adding different concentrations of cerivastatin, the eNOS mRNA was detected by RT-PCR and the amount of NO in the medium by nitrate reductase. After adding lipopolysaccharide (LPS) and cerivastatin into ECV-304 cells, ICAM-1 mRNA was detected by RT-PCR and the amount of adherent THP1 cells was measured. Results With the increase of cerivastatin concentration, the level of eNOS mRNA in endothelial cells increased by 50%, 150%, 300% respectively at 0 0 1, 0 1, 1 0 μmol / L, P <0 0 5. In the culture medium, the NO level also increased correspondingly, with the increase of 267%, 92 3% and 230%, respectively, at 0 0 1, 0 1 and 10 μmol / L, P <0 05. ICAM 1 mRNA decreased from 70 7 ± 1 0 4 to 5 6 2 ± 6 5 and 3 5 8 ± 4 2, respectively (P <0 05) when the concentration of cerivastatin was 0 1 and 10 μmol / L. THP1 cells adhering to ECV340 cells were inhibited by 26% at 10 μmol / L, P <0 05. Conclusion Cerivastatin can induce endothelial eNOS gene expression and increase NOS activity; inhibit the expression of ICAM 1 mRNA in endothelial cells and adhesion of THP 1 cells to endothelial cells