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目的 了解合肥地区献血员的庚型肝炎病毒 (GBV C/HGV)感染情况。方法 应用酶免疫测定法(EIA)和逆转录 套式多聚酶链反应法 (RT PCR)分别检测献血员中的抗 GBV C/HGV和GBV C/HGVRNA。结果 献血员中抗 GBV C/HGV检出率为 1 7% (18/10 50 ) ,抗 GBV C/HGV阳性血清中GBV C/HGVRNA占 66 7% (12 /18) ;男性和低年龄组献血员抗 GBV C/HGV阳性率分别低于女性和高年龄组 ,两者差异有显著性 (P <0 0 5) ;抗 GBV C/HGV阳性与献血次数多少、献血时间长短、ALT水平、HBV和 (或 )HCV感染无关 ;对 2 0 0名献血员进行 3个月追踪 ,198名抗 GBV C/HGV阴性的献血员有 6名转为阳性 ,2名抗 GBV C/HGV和GBV C/HGVRNA阳性的献血员有 1名转为阴性。结论 GBV C/HGV在合肥地区献血员中有较高的感染率 ;献血员中存在着GBV C/HGV阳性但ALT正常的献血员 ,应尽快对献血员进行GBV C/HGV感染指标的检测。
Objective To understand the prevalence of hepatitis G virus (GBV C / HGV) infection among blood donors in Hefei area. Methods Anti-GBV C / HGV and GBV C / HGVRNA in blood donors were detected by enzyme-linked immunosorbent assay (EIA) and reverse transcription polymerase chain reaction (RT PCR). Results The detection rate of anti-GBV C / HGV in blood donors was 17% (18/10 50), that of GBV C / HGV-positive serum was 66 7% (12 of 18) The positive rates of anti-GBV C / HGV in blood donors were lower than those in female and high age groups (P <0.05), the number of anti-GBV C / HGV positive and blood donation, the duration of blood donation, the level of ALT, HBV and / or HCV infection; 3 months follow-up of 200 blood donors; 6 of the 198 anti-GBV C / HGV-negative blood donors turned positive; 2 anti-GBV C / HGV and GBV C One of the HGV RNA positive donors turned negative. Conclusion GBV C / HGV has a higher infection rate among blood donors in Hefei. There are blood donors who have positive GBV C / HGV but normal ALT in blood donors, and blood samples should be tested for GBV C / HGV infection as soon as possible.