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以多只毛蚴感染多只钉螺后，逐周取样３０只，以抗血吸虫卵的ＭＧ２ＭｃＡｂ，应用ＥＬＩＳＡ检测螺体内血吸虫抗原，并同时以镜检为对照。结果表明，感染３周阳性钉螺体内血吸虫抗原的阳性检出率为７０％，以后逐周提高，５周为９０％，８周为９６．６７％。提示该法似有早期鉴别阳性钉螺的效能。在ＥＬＩＳＡ检测不同数量毛蚴感染钉螺体内血吸虫抗原阳性率的比较中，感染后期（８周），多只毛蚴感染组螺体内抗原阳性检出率为９６．６７％；单只毛蚴感染组为１００％，两组基本相似。结果表明，不论单只毛蚴或多只毛蚴感染钉螺，在感染后期，用ＥＬＩＳＡ检测螺体内血吸虫抗原的阳性率无差异。
After infecting more than one snail with more than one miracidium, 30 samples were taken weekly to detect the MG2McAb of the schistosome eggs. ELISA was used to detect the schistosome antigen in the spirochetes, and microscopic examination was used as the control. The results showed that the positive detection rate of schistosoma antigen in positive snails was 70% at 3 weeks after infection, and then increased weekly, 90% at 5 weeks and 96.67% at 8 weeks. Suggesting that the law seems to identify early positive snail performance. In the later stage of infection (8 weeks), the positive rate of positive antigen in spirochaete was 96.67% in the multiple infection groups and 100% in the single infection group The two groups are basically similar. The results showed that there was no difference in the positive rate of schistosoma antigen in the spirochetes detected by ELISA in the later stage of infection, no matter whether there were only one miracidia or more miracidia infected with Oncomelania hupensis.