作者将三种抗麻疹病毒融合(F)蛋白的单克隆抗体(McAb)和一种抗血凝素(H)蛋白的McAb经生物素标记并用于生物素-链霉亲和素酶免疫试验(EIA)系统,进行了以下几项试验: 1.用未标记的上述4种McAb作为竞争抗体,分别阻断相应的标记McAb与抗原的结合,在25%的抑制水平判定滴度。本法可分别测出麻疹病毒F蛋白和H蛋白抗体,没有交叉反应,并能区分出F多肽上至少有两个 The authors biotinylated the three monoclonal antibodies to the anti-measles virus fusion (F) protein and a McAb against the hemagglutinin (H) protein and used it for biotin-streptavidin immunoassay EIA) system, the following tests were carried out: 1. Using the unlabeled McAb as a competitive antibody, respectively, to block the binding of the corresponding labeled McAb to the antigen, and to determine the titer at the level of 25% inhibition. This method can be measured measles virus F protein and H protein antibodies, no cross-reaction, and can distinguish at least two F polypeptide