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目的探讨姜黄素对脊髓压迫性损伤脱髓鞘病变的保护作用。方法采用自行设计的压迫装置制作压迫性脊髓损伤模型。用30只成年SD大鼠,雌雄不拘,随机分为假手术组(10只),模型组(10只)和治疗组(10只)。治疗组,于脊髓压迫2h后,减压,并腹腔注射姜黄素(100 mg/kg)进行干预性治疗,而于假手术组、模型组则注射0.1%PBS进行对照。运用BBB(Basso BeattieBresnahan)评分观察动物神经功能的恢复情况;应用Luxol fast blue(LFB)、锇酸染色和透射电镜等方法 ,观察姜黄素处理前、后脊髓白质纤维髓鞘变化情况。结果 24 h后,模型组大鼠白质有髓神经纤维出现水肿、排列疏松及髓鞘缺失等退行性变,其纤维数目逐渐减少,与假手术组相比有统计学意义(P<0.05)。应用姜黄素后,动物行为学指标有所改善,BBB评分升高,与模型组相比较,差异具有统计学意义(P<0.05);其白质髓鞘化神经纤维水肿、排列疏松等病理变化有所减轻,其髓鞘化纤维计数较模型组增多,与模型组相比具有统计学意义(P<0.05)。结论姜黄素对脊髓压迫性损伤脱髓鞘病变有保护作用。
Objective To investigate the protective effect of curcumin on demyelinating lesions of spinal cord injury. Methods Compression-induced spinal cord injury model was made by self-designed compression device. Thirty adult SD rats were randomly divided into sham operation group (n = 10), model group (n = 10) and treatment group (n = 10). The rats in the treatment group were treated with curcumin (100 mg / kg) intraperitoneally for 2 hours after spinal cord compression. In the sham operation group, 0.1% PBS was injected into the model group. The BBB (Basso Beattie Bresnahan) score was used to observe the recovery of neurological function in the animals. Luxol fast blue (LFB), osmium tetroxide staining and transmission electron microscopy were used to observe the changes of myelin in the white matter fibers of the spinal cord before and after curcumin treatment. Results After 24 h, the white matter myelinated nerve fibers of rats in the model group showed degenerative changes such as edema, loose arrangement and demyelination. The number of the fibers gradually decreased, which was statistically significant compared with the sham operation group (P <0.05). After application of curcumin, animal behavioral indicators improved, BBB score increased, compared with the model group, the difference was statistically significant (P <0.05); the white matter myelinated nerve fiber edema, arranged in loose and other pathological changes Compared with the model group, the count of myelinated fibers increased significantly compared with the model group (P <0.05). Conclusion Curcumin has a protective effect on demyelinating lesions of spinal cord compression injury.