Eukaryotic Expression and Activity Analysis of Capsid Gene of Swine Vesicular Disease Virus HK/70

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The capsid protein precursor(P1),which plays a major role for the generation of polypeptides of swine vesicular disease virus(SVDV),was cloned from SVDV HK/70 strain into the retroviral vector pBABE puro and expressed in the mammalian cell line PK15 through the retroviral expression system.The activity of recombinant protein to induce immune response was evaluated in guinea pigs.IFA and West Blot were used to detect the recombinant protein expression.The results showed that the recombinant protein could be recognized by SVDV positive serum,and animal test showed SVDV-specific antibodies.All of those results indicate that a retroviral-based vaccine carrying the capsid protein precursor(P1)of SVD is able to be expressed in the eukaryotic cell and elicites strong SVDV-specific immune responses in guinea pigs.
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