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本文试验了采用原子吸收分光光度计的光路系统测量分子光谱的可行性．在ＰＨ＝３．５的ＨＡＣ－ＮａＡｃ介质中，在避光条件下，钴对无色的Ｆｅ（Ⅲ）－联吡啶配合物还原成红色的Ｆｅ（Ⅱ）－联吡啶配合物有较强的催化作用．在λ＝５２２９ｎｍ处，在原子吸收分光光度计上测定钴的含量，对钴测定的线性范围为３～１５０μｇ／２５ｍｌ，检测限力０．０３８μｇ／２５ｍｌ．本法选择性较好，多数离子允许大量存在，将此法用于维生素Ｂ１２针剂中钴的测定，并与分光光度法及原子吸收分光光度法对照．结果令人满意
This article tested the feasibility of measuring molecular spectra using an optical system of an atomic absorption spectrophotometer. In the HAC-NaAc medium with pH = 3.5, the cobalt (Ⅱ) -bipyridine complex reduced to colorless Fe (Ⅲ) -bipyridine complex to red Catalysis. At λ = 5229 nm, the cobalt content was determined on an atomic absorption spectrophotometer with a linear range of 3 to 150 μg / 25 ml for cobalt and a limit of detection of 0.038 μg / 25 ml for cobalt. This method is better selectivity, most of the ions allowed to exist in large quantities, this method is used for the determination of cobalt in vitamin B12 injection, and compared with spectrophotometry and atomic absorption spectrophotometry. The result is satisfactory