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本文报道了增加脂质体体外稳定性的实验研究,我们分别选用了甘露醇加聚乙烯硫酸酯(Poeylinyl Sulfate,简称PVS)、右旋糖苷、猪肝胞质RNA作为脂质体的稳定因子。通过4℃冰箱存放至100天或用丙酮—干冰低温加速破坏后经电镜观察以及超滤法除去凝聚,融合的大颗粒放射性脂质体等方法均显示,只要脂质体溶液中存在甘露醇—PVS,其脂质体的形态及残留于超滤膜上的放射强度测定均较生理盐水对照组及其它各组稳定。 经统计学处理有显著性意义,P值小于0.05或0.01。
In this paper, we report the experimental study on increasing the in vitro stability of liposomes. We selected mannitol and PVS, dextran and porcine liver cytoplasmic RNA as the stable factors of liposome respectively. After being stored in a refrigerator at 4 ° C for 100 days or accelerated by acetone-dry ice at low temperature and then destroyed by electron microscopy and ultrafiltration to remove agglomeration and fusion of large-particle radioactive liposomes, etc., the results showed that as long as the mannitol- PVS, its liposome morphology and remaining in the ultrafiltration membrane radiation intensity determination than the saline control group and other groups were stable. After statistical treatment was significant, P value less than 0.05 or 0.01.