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目的了解黑龙江省边境口岸地区健康儿童肠道病毒(EV)携带状况。方法采取单纯随机抽样方法,在19个边境口岸地区共采集798份健康儿童粪便标本,用RD、L20B和Hep-2细胞分离病毒,用微量板法中和试验进行鉴定。结果在798份粪便标本中检出EV 132株,阳性率为16.54%。在132株EV中,脊髓灰质炎(脊灰)病毒26株,其中4株为脊灰疫苗高变异株病毒;非脊灰肠道病毒(NPEV)106株。结论黑龙江省边境口岸地区健康儿童EV携带较为普遍,未发现脊灰野病毒。
Objective To understand the carrying status of enterovirus (EV) in healthy children at border crossings in Heilongjiang Province. Methods A total of 798 stool samples from healthy children were collected from 19 border crossing areas by using a simple random sampling method. Viruses were isolated from RD, L20B and Hep-2 cells and identified by microtitre plate neutralization assay. Results EV 132 strains were detected in 798 stool specimens, the positive rate was 16.54%. Among the 132 EVs, 26 were poliomyelitis (poliovirus), of which 4 were high-mutant poliovirus and 106 were non-polio enterovirus (NPEV). Conclusion Hepatitis C virus is more common in healthy children at the border crossings in Heilongjiang Province. No poliovirus was found.