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本文对六个健康个体骨髓细胞姊妹染色单体互换(SCE)进行了研究,与外周血淋巴细胞比较,SCE显著的低而恒定。每个中期分裂相变动范围为3.64～4.65。骨髓细胞得自白血病患者的兄妹(三个男人和三个女人),在加有20％小牛血清的培养基RPM I 1640中培养50小时,不加PHA。培养时间或长或短,即呈现一个可估价的百分率较低的中期分裂相,5-溴脱氧尿苷(BudR)最终浓度为10～15μM。在培养终止前2小时加秋水酰胺(0.02μg/
In this paper, the sister chromatid exchange (SCE) of bone marrow cells in six healthy individuals was studied. Compared with peripheral blood lymphocytes, SCE was significantly lower and constant. The range of change for each metaphase is 3.64 to 4.65. Bone marrow cells were obtained from brothers and sisters of leukemia patients (three men and three women) and cultured in medium RPMI 1640 supplemented with 20% calf serum for 50 hours without PHA. The culture time was either long or short, ie, it showed a measurable percentage of the intermediate metaphase and the final concentration of 5-bromodeoxyuridine (BudR) was 10-15 μM. Plasmodamide (0.02 μg/kg) was added 2 hours before the termination of culture.