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对12例高危白血病患者进行氮介(NM)和足叶乙甙(VP-16)净化的PBSC自体移植,以评估对动员的干细胞实施剂量个体化药物净化的可行性。12例患者,男5例,女7例,中位年龄46(18～57)岁。AML9例,ALL2例,套细胞淋巴瘤伴白血病播散(NHL/Leuk)1例,均为高危患者。6例患者用氟哒拉平(FLAN)+G-CSF作动员方案,3例患者用NOVIA方案(中剂量Ara-C+米托蒽醌)+G-CSF作动员方案,2例ALL和1例AML患者单用G-CSF动员干细胞。以化疗加G-CSF动员的患者在CD34~+细胞>10000/μl时采集,单用G-CSF者在第5天采集,用Fenwal C83000或Cobe Spectra分离干细胞,使CD34~+细胞≥2×10~6/kg。体外净化时VP-16放置5ml安瓿中
Twenty-two high-risk leukemia patients were treated with PBSC autotransplantation with nitrogen-mediated (NM) and etoposide (VP-16) decontamination to assess the feasibility of dose-based individualized drug decontamination of mobilized stem cells. In 12 patients, there were 5 males and 7 females, with a median age of 46 (18-57) years. 9 cases of AML, 2 cases of ALL, and 1 case of mantle cell lymphoma with disseminated leukemia (NHL/Leuk) were all high-risk patients. Fifty patients (FLAN) + G-CSF were used as mobilization protocol in 6 patients, 3 patients were treated with NOVIA (medium dose Ara-C + mitoxantrone) + G-CSF as mobilization protocol, 2 patients with ALL and 1 patient with AML The patient mobilized stem cells with G-CSF alone. Patients treated with chemotherapy plus G-CSF were collected at CD34~+ cells > 10000/μl. G-CSF alone was collected on day 5, and stem cells were isolated with Fenwal C83000 or Cobe Spectra, making CD34~+ cells ≥ 2 × 10~6/kg. VP-16 is placed in 5ml ampoules during in vitro purification