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目的：探讨束缚应激对肝脏S-腺苷甲硫氨酸（SAM）和S-腺苷同型半胱氨酸（SAH）代谢的影响。方法Wistar大鼠16只随机均分为对照组和束缚应激组，束缚组每天束缚6 h，束缚6周。然后处死大鼠，用高效液相色谱法测定肝组织中SAM、SAH和S-腺苷同型半胱氨酸水解酶（SAHH）的活性。以SAH单位时间生成量和减少量来计算该酶的合成活性和水解活性。结果束缚组大鼠肝脏SAM含量显著低于对照组（P＜0.05），而SAH含量却显著高于对照组（P＜0.05），束缚组SAM/SAH显著低于对照组（P＜0.05），两组SAHH合成活性间差异无统计学意义（P＞0.05），但束缚组SAHH水解活性显著低于对照组（P＜0.05）。结论慢性束缚应激可能抑制机体转甲基作用。“,”Objective To investigate the effects of chronic restraint stress on S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) metabolism. Methods Sixty male Wistar rats were randomly divided into two equal groups control group and restraint stress (RS) group undergoing RS 6h daily for 6 weeks. Then the rats were killed with their livers taken oot. High performance liquid chromatograph was used to detect the concentrations of SAM and SAH. The increment of SAM in unit time was calculated as the synthetic activity of liver S-adenosylhomocysteine hydrolase (SAHH) and the cecrement in unit time was calculated as the hydrolytic activity of SAHH. Results The concentration of SAM of the RS group was significantly lower than that of the contgrol gtoup (P<0.05) and concentration of SAH of the RS group was significantly higher than that of the control group (P<0.05). The SAM/SAH ratio of the RS group was significantly lower than that of the control group (P0.05), however, the SAHH hydrolytic activity of the RS group was significantly lower than that of the control grouip (P<0.05). Conclusion Chronic stress inhabits the tans-methylation pathway in the organisms.