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目的建立高效液相色谱-串联质谱法同时测定果蔬中26种植物生长调节剂残留量的检测方法。方法样品经QuEChERS法预处理,用含1%甲酸的乙腈溶液提取,无水硫酸镁和十八烷基硅烷(C_(18))粉末净化,以Agilent Poroshell 120 EC-C_(18)(3.0 mm×100 mm,2.7μm)为分析色谱柱,甲醇和5 mmol/L乙酸铵-0.1%甲酸缓冲液作为流动相进行梯度洗脱分离,采用电喷雾多反应监测模式,基质匹配标准溶液外标法定量。结果 26种植物生长调节剂线性良好,线性相关系数均>0.990,该方法的检出限在1~10μg/kg之间,以豆芽和苹果为代表性基质进行3个水平加标回收实验,回收率在80.81%~118.4%之间,相对标准偏差(RSD)为0.9%~14.8%。结论该方法灵敏度高,定性、定量准确,简便高效,可适用于多种水果和蔬菜中26种植物生长调节剂残留的同时检测要求。
Objective To establish a method for the simultaneous determination of 26 plant growth regulators residues in fruits and vegetables by high performance liquid chromatography-tandem mass spectrometry. Methods The samples were pretreated by QuEChERS method, extracted with acetonitrile containing 1% formic acid and cleaned up with anhydrous magnesium sulfate and octadecylsilane (C_ (18)). The samples were purified with Agilent Poroshell 120 EC-C 18 × 100 mm, 2.7 μm) were separated by gradient elution with methanol, 5 mmol / L ammonium acetate-0.1% formic acid buffer as the mobile phase. Electrospray ionization multiple reaction monitoring Quantitative. Results 26 kinds of plant growth regulators had good linearity and the linear correlation coefficients were all> 0.990. The detection limit of this method was between 1 ~ 10μg / kg. The sprouts and apples were taken as the representative matrix to carry out spike recovery experiment Rates ranged from 80.81% to 118.4% with relative standard deviations (RSDs) of 0.9% to 14.8%. Conclusion The method is sensitive, qualitative, accurate, simple and efficient and can be applied to the simultaneous detection of 26 plant growth regulators residues in many fruits and vegetables.