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本实验目的是观察模拟失重与高浓度氧暴露12—36h对大鼠肺脏的影响,研究两者的复合效应.实验共用Wistar大鼠60只,分为5个组.尾悬吊(-30°)作为模拟失重动物模型.结果:单纯高氧暴露12h肺组织仅见炎性细胞浸润,36h部分内皮细胞的内质网发生肿胀,密度降低,线粒体密度增加,结构模糊.与单纯吸氧组相比,悬吊+高浓度氧对大鼠肺脏的复合效应除有上述表现外,主要表现为肺水肿出现早且较重,肺泡上皮细胞、内皮细胞和基底膜均有水肿.高氧及悬吊+高氧两组肺泡灌洗液中二棕榈酸磷脂酰胆硷(DSPC)均为12h减少,36h回升,其表面张力均增高.血清中血管紧张素(ACE)活性也为先降低后回升.这些结果表明两种因素复合作用时肺脏的损伤加重.从这个意义上说,地面条件下氧的中毒剂量单位和耐限须经过模拟失重的修正才能用于航天.
The purpose of this experiment was to observe the effect of simulated weightlessness and high concentration oxygen exposure 12-36h on the lungs of rats and to study the compound effect of the two.A total of 60 Wistar rats were divided into 5 groups.The tail suspension ) Were used as model animals to simulate weightlessness.Results: Only the inflammatory cell infiltration was observed in the lungs exposed to hyperoxia for 12h, the endoplasmic reticulum of some endothelial cells swelled, the density decreased, the density of mitochondria increased and the structure was blurred at 36h.Compared with the pure oxygen group , The compound effect of suspension + high concentration of oxygen on rat lungs except for the above performance, mainly as early and heavier pulmonary edema, alveolar epithelial cells, endothelial cells and basement membrane edema .High oxygen and suspension + The levels of phosphotidyl palmitate (DSPC) and dipalmitoyl phosphatidylcholine (DSPC) in the two groups of alveolar lavage fluid decreased 12h and 36h after resuscitation, respectively, and their surface tension increased, while the activity of ACE in serum decreased first and then rose. The results showed that the combination of the two factors lung injury increased.In this sense, under the ground conditions oxygen poisoning dose units and resistance to be modified by simulated weight loss can be used for spaceflight.