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本文对抗枯萎病性不同的12个陆地棉品种胚轴外植体进行了培养、比较,并就枯萎菌毒素对其愈伤组织诱导的影响进行了研究。发现,(1)12个品种胚轴外植体在 Ls+IAA 2mg/L+KT 1mg/L 培养基上,均能产生胚性和非胚性两类不同性质的愈伤组织;(2)枯萎菌毒素提取液经高压灭菌后,仍具有较强的毒害活力;(3)枯萎菌病毒素提取液对陆地棉胚轴外植体具有很强的毒害作用,从而抑制了胚轴外植体愈伤组织的产生,且随浓度增加而作用愈强;(4)抗病性不同的品种对枯萎菌毒素的反应情况不同,高抗品种受毒害较轻,且能产生较多的愈伤组织,而高感品种受害较重,且很难形成愈伤组织;(5)采用逐代提高毒素浓度的培养方法,对愈伤组织无性系进行继代筛选,获得了少量抗高浓度毒素的愈伤组织无性系。这些结果为进一步通过组织细胞培养,获得抗病植株及试管快速鉴定抗病品种等工作奠定了基础。
In this paper, the hypocotyl explants of 12 Gossypium hirsutum cultivars with different resistance to fusarium wilt were cultured and compared, and the effects of X. fragilis toxin on their callus induction were studied. The results showed that: (1) 12 kinds of hypocotyl explants could produce two types of embryogenic and non-embryogenic calluses with Ls + IAA 2mg / L and KT 1mg / L respectively; (2) Fusarium mycotoxin extract still has strong toxic activity after being autoclaved; (3) The extract of Fusarium oxysporum virulentum has a strong toxic effect on the hypocotyl explants of G. hirsutum, thus inhibiting the hypocotyls explants Body callus, and stronger with increasing concentration. (4) The response of different resistant varieties to Fusarium mycotoxins was different, and the high resistant varieties were less toxic and could produce more callus (5) The subculture of clones of calli was subcultured by increasing the concentration of toxin, and a small amount of high concentration of toxin was obtained Callus clones. These results lay a foundation for further work on tissue culture, obtaining resistant plants and rapid identification of resistant varieties in vitro.